My project looked at the effects of anti-cancer drugs on primary cilia-associated PDGFR-alpha signaling. Primary cilia house many receptors that are important for the regulation of normal cell signaling. In cancer, ciliation is often abnormal as is cell signaling. I wanted to see if there is a relationship between cell signaling and ciliation state. My lab previously found that a number of clinically available anti-cancer drugs do have an effect on ciliation, either promoting their growth or removal. I hypothesized that cells treated with drugs that promoted ciliation would have higher levels of PDGFRa signaling while cilia destabilizing drugs would reduce signaling. I cultured NIH cells, serum starved them to induce ciliation, and treated them with the drugs of interest. I then analyzed ciliation state through immunofluorescence, and PDGFRa signaling through Western blotting and immunofluorescence. I found that by inhibiting ciliation, there is a reduction in PDGRa signaling.