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Aman Sharma

Member of The Honor Society of Phi Kappa Phi
From UMass Amherst, MA
PhD Candidate at the University of Massachusetts Amherst. Pursuing PhD in Animal Biotechnology and Biomedical Science program from Veterinary and Animal Science department in Dr. D. Joseph Jerry lab. Seeking an opportunity for effective research in the field of biological sciences and be a part in a motivated team working on challenging projects. Currently working in the molecular mechanisms of breast cancer induced by estrogen and studying the effect of xenoestrogens in mammary gland biology has harnessed my skills. I would be thrilled to utilize my existing skills in developmental and molecular biology along with my experience in cancer biology to excel in the research while attaining new skills in the emerging technology to establish as a research scientist in future. I have a Bachelor's degree (4 years) of B.Tech in Industrial Biotechnology and research experience from my summer internship and dissertation.
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Phi Kappa Phi Announces Graduate Research Grant Recipients

The Honor Society of Phi Kappa Phi, the nation's oldest and most selective all-discipline collegiate honor society, today announced the recipients of its Graduate Research Grants. The grants of up to $1,500 are designed to support graduate students who...

March, 11 2021 - Verified by The Honor Society of Phi Kappa Phi
Aman Sharma Awarded Phi Kappa Phi Graduate Research Grant

Aman Sharma, of Amherst, Massachusetts, has been selected as a 2021 recipient of a Graduate Research Grant by The Honor Society of Phi Kappa Phi-the nation's oldest and most selective collegiate ho...

March, 11 2021 - Verified by The Honor Society of Phi Kappa Phi
Aman Sharma Receives 2020 Phi Kappa Phi Love of Learning Award

Aman Sharma was recently awarded a Love of Learning Award worth $500 from The Honor Society of Phi Kappa Phi, the nation's oldest and most selective collegiate honor society for all academic discip...

October, 05 2020 - Verified by The Honor Society of Phi Kappa Phi
Aman Sharma Inducted into The Honor Society of Phi Kappa Phi

Aman Sharma of Sunderland, Massachusetts, was recently initiated into The Honor Society of Phi Kappa Phi, the nation's oldest and most selective all-discipline collegiate honor society. Sharma was...

April, 08 2020 - Verified by The Honor Society of Phi Kappa Phi
Inter-Individual Variation in Response to Estrogen in Human Breast Explants
Exposure to estrogen is strongly associated with increased breast cancer risk. While all women are exposed to estrogen, only 12% are expected to develop breast cancer during their lifetime. These women may be more sensitive to estrogen, as rodent models have demonstrated variability in estrogen sensitivity. Our objective was to determine individual variation in expression of estrogen receptor (ER) and estrogen-induced responses in the normal human breast. Human breast tissue from female donors undergoing reduction mammoplasty surgery were collected for microarray analysis of ER expression. To examine estrogen-induced responses, breast tissue from 23 female donors were cultured ex- vivo in basal or 10 nM 17β-estradiol (E2) media for 4 days. Expression of ER genes (ESR1 and ESR2) increased significantly with age. E2 induced consistent increases in global gene transcription, but expression of target genes AREG, PGR, and TGFβ2 increased significantly only in explants from nulliparous women. E2-treatment did not induce consistent changes in proliferation or radiation induced apoptosis. Responses to estrogen are highly variable among women and not associated with levels of ER expression, suggesting differences in intracellular signaling among individuals. The differences in sensitivity to E2-stimulated responses may contribute to variation in risk of breast cancer.
March 2020 - Publications
Effects of Benzophenone-3 and Propylparaben on Estrogen Receptor–Dependent R-Loops and DNA Damage in Breast Epithelial Cells and Mice
Background: Endocrine-disrupting chemicals have been shown to have broad effects on development, but their mutagenic actions that can lead to cancer have been less clearly demonstrated. Physiological levels of estrogen have been shown to stimulate DNA damage in breast epithelial cells through mechanisms mediated by estrogen-receptor alpha (ERα). Benzophenone-3 (BP-3) and propylparaben (PP) are xenoestrogens found in the urine of >96% of U.S. population. Objectives: We investigated the effect of BP-3 and PP on estrogen receptor–dependent transactivation and DNA damage at concentrations relevant to exposures in humans. Methods: In human breast epithelial cells, DNA damage following treatment with 17β-estradiol (E2), BP-3, and PP was determined by immunostaining with antibodies against γ-H2AX and 53BP1. Estrogenic responses were determined using luciferase reporter assays and gene expression. Formation of R-loops was determined with DNA: RNA hybrid–specific S9.6 antibody. Short-term exposure to the chemicals was also studied in ovariectomized mice. Immunostaining of mouse mammary epithelium was performed to quantify R-loops and DNA damage in vivo. Results: Concentrations of 1μM and 5μM BP-3 or PP increased DNA damage similar to that of E2 treatment in a ERα-dependent manner. However, BP-3 and PP had limited transactivation of target genes at 1μM and 5μM concentrations. BP-3 and PP exposure caused R-loop formation in a normal human breast epithelial cell line when ERα was introduced. R-loops and DNA damage were also detected in mammary epithelial cells of mice treated with BP-3 and PP. Conclusions: Acute exposure to xenoestrogens (PP and BP-3) in mice induce DNA damage mediated by formation of ERα-dependent R-loops at concentrations 10-fold lower than those required for transactivation. Exposure to these xenoestrogens may cause deleterious estrogenic responses, such as DNA damage, in susceptible individuals.
January 2020 - Publications

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